Hotspot

psaA

Genome · 131183-133441 · 2259 bp

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Pi 0.123
MarkerSeek score 80.80
Primer available yes
Label psaA

Feature Metadata

label_name
psaA
feature_type
gene
region
Genome
length_bp
2259
variable_sites
278
pi
0.123
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA_p1 TCCTTGTCTCAAGAGAGGAGA AAGCTTGGCTAAACTTAGGA 47.6 40.0 57.2 54.2 2346-2347 bp 1.0 48.0
psaA_p2 TCCTTGTCTCAAGAGAGGAGA AGCTTGGCTAAACTTAGGAA 47.6 40.0 57.2 54.2 2345-2346 bp 1.0 48.0
psaA_p3 GCGTTTTGATTCCTTGTCTCA AAGCTTGGCTAAACTTAGGA 42.9 40.0 57.1 54.2 2356-2357 bp 1.0 48.0
psaA_p4 GCGTTTTGATTCCTTGTCTCA AGCTTGGCTAAACTTAGGAA 42.9 40.0 57.1 54.2 2355-2356 bp 1.0 48.0
psaA_p5 TCCTTGTCTCAAGAGAGGAGA AGCTTGGCTAAACTTAGGAAA 47.6 38.1 57.2 55.1 2345-2346 bp 1.0 48.0