Hotspot

orf745

Genome · 80524-82668 · 2145 bp

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Pi 0.357
MarkerSeek score 78.90
Primer available yes
Label orf745

Feature Metadata

label_name
orf745
feature_type
gene
region
Genome
length_bp
2145
variable_sites
753
pi
0.357
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
orf745_p1 GCCTCTGACTCTCCATTTGG TCCGAGCAAAAAGATACTGCA 55.0 42.9 58.0 57.9 2947 bp 0.5 51.3
orf745_p2 GCCTCTGACTCTCCATTTGG TCCACTTTCCTTTGCTACGA 55.0 45.0 58.0 56.8 2556 bp 0.5 50.0
orf745_p3 TTTAACGGCATTGGCACTCT TCCACTTTCCTTTGCTACGA 45.0 45.0 58.1 56.8 2899 bp 0.5 49.5
orf745_p4 GGCATTGGCACTCTCATTTG TCCACTTTCCTTTGCTACGA 50.0 45.0 57.7 56.8 2893 bp 0.5 48.0
orf745_p5 GCATTGGCACTCTCATTTGC TCCACTTTCCTTTGCTACGA 50.0 45.0 58.4 56.8 2892 bp 0.5 47.4