Hotspot

orf286

Genome · 85006-85866 · 861 bp

Back to Bangia

Pi 0.316
MarkerSeek score 78.80
Primer available yes
Label orf286

Feature Metadata

label_name
orf286
feature_type
gene
region
Genome
length_bp
861
variable_sites
272
pi
0.316
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
orf286_p1 CATGACCGAAGTCGTCACAT GACGCTTTAAATGTGCCCAC 50.0 50.0 58.0 58.0 1380 bp 0.5 59.8
orf286_p2 AAAGTTCCGGTTGCTCCAAT GACGCTTTAAATGTGCCCAC 45.0 50.0 58.0 58.0 1497 bp 0.5 59.8
orf286_p3 AAGTTCCGGTTGCTCCAATT GACGCTTTAAATGTGCCCAC 45.0 50.0 58.0 58.0 1496 bp 0.5 59.8
orf286_p4 GAACCCTCATGACCGAAGTC GACGCTTTAAATGTGCCCAC 55.0 50.0 58.0 58.0 1387 bp 0.5 59.7
orf286_p5 TGCGTATACCAATTTCGCCA GACGCTTTAAATGTGCCCAC 45.0 50.0 58.0 58.0 1349 bp 0.5 59.6