Hotspot

ntcA

Genome · 178350-178952 · 603 bp

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Pi 0.26
MarkerSeek score 78.80
Primer available yes
Label ntcA

Feature Metadata

label_name
ntcA
feature_type
gene
region
Genome
length_bp
603
variable_sites
157
pi
0.26
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ntcA_p1 TCAACCAGCCAAAGTTGACA GCTGGTGATGGATTCTCTCG 45.0 55.0 57.9 58.1 1527 bp 0.5 57.8
ntcA_p2 TTTCTCCAACAACAGGCACA GCTGGTGATGGATTCTCTCG 45.0 55.0 57.9 58.1 1773 bp 0.5 57.8
ntcA_p3 TCAACCAGCCAAAGTTGACA GGCTGGTGATGGATTCTCTC 45.0 55.0 57.9 57.7 1528 bp 0.5 56.9
ntcA_p4 TTTCTCCAACAACAGGCACA GGCTGGTGATGGATTCTCTC 45.0 55.0 57.9 57.7 1774 bp 0.5 56.9
ntcA_p5 TAAAATCACGCCGGTTACCC GCTGGTGATGGATTCTCTCG 50.0 55.0 58.3 58.1 1655 bp 0.5 56.8