Hotspot

fabH

Genome · 52511-53491 · 981 bp

Pi 0.178

MarkerSeek score 79.20

Primer available yes

Label fabH

Feature Metadata

label_name: fabH
feature_type: gene
region: Genome
length_bp: 981
variable_sites: 174
pi: 0.178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
fabH_p1	AGTCAACTCAAGCCCGAAAG	ACAGCCCCAAGGATAAGACT	50.0	50.0	58.1	58.0	1584 bp	0.5	58.9
fabH_p2	GCTGCAGGTTCACGAAAAAG	ACAGCCCCAAGGATAAGACT	50.0	50.0	58.2	58.0	1904 bp	0.5	57.9
fabH_p3	AACCTTGTCAGAACCGGAAG	ACAGCCCCAAGGATAAGACT	50.0	50.0	57.7	58.0	1545 bp	0.5	57.7
fabH_p4	AGTCAACTCAAGCCCGAAAG	ACTCGATAAGGACGGTGTCA	50.0	50.0	58.1	58.2	1555 bp	0.5	57.7
fabH_p5	TTTACGCGATTATGGCTGGT	ACAGCCCCAAGGATAAGACT	45.0	50.0	57.7	58.0	1875 bp	0.5	57.2