Hotspot

chlN

Genome · 46091-47416 · 1326 bp

Pi 0.176

MarkerSeek score 79.30

Primer available yes

Label chlN

Feature Metadata

label_name: chlN
feature_type: gene
region: Genome
length_bp: 1326
variable_sites: 233
pi: 0.176
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN_p1	GCTAGGCCAGAAGGAGTAGT	TTGGCGGATTAGTTGGTGAG	55.0	50.0	58.2	57.9	2143 bp	0.5	57.4
chlN_p2	TGCTAGGCCAGAAGGAGTAG	TTGGCGGATTAGTTGGTGAG	55.0	50.0	58.2	57.9	2144 bp	0.5	57.4
chlN_p3	TTGCTAGGCCAGAAGGAGTA	TTGGCGGATTAGTTGGTGAG	50.0	50.0	57.8	57.9	2145 bp	0.5	57.2
chlN_p4	GATTGCTAGGCCAGAAGGAG	TTGGCGGATTAGTTGGTGAG	55.0	50.0	57.7	57.9	2147 bp	0.5	57.1
chlN_p5	GCTAGGCCAGAAGGAGTAGT	AAGTGGCGCTAGAGCAATTT	55.0	45.0	58.2	58.2	1660 bp	0.5	56.9