Hotspot

rps1-syfB

Genome · 180951-181490 · 540 bp

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Pi 0.171
MarkerSeek score 79.30
Primer available yes
Label rps1-syfB

Feature Metadata

label_name
rps1-syfB
feature_type
igs
region
Genome
length_bp
540
variable_sites
91
pi
0.171
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps1-syfB_p1 TGCTGATACAGGTGTACCGA GGTCTAAACCCGCACATTCT 50.0 50.0 58.2 57.9 1298 bp 0.5 57.9
rps1-syfB_p2 TGCTGATACAGGTGTACCGA TGGTCTAAACCCGCACATTC 50.0 50.0 58.2 58.2 1299 bp 0.5 57.3
rps1-syfB_p3 TCTTCAGCGAGCAATTGTCT GGTCTAAACCCGCACATTCT 45.0 50.0 57.5 57.9 1483 bp 0.5 55.4
rps1-syfB_p4 TGCTGATACAGGTGTACCGA GTGGTCTAAACCCGCACATT 50.0 50.0 58.2 58.5 1300 bp 0.5 55.0
rps1-syfB_p5 TCTTCAGCGAGCAATTGTCT TGGTCTAAACCCGCACATTC 45.0 50.0 57.5 58.2 1484 bp 0.5 54.8