primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
chlN_p1	chlN	1	GCTAGGCCAGAAGGAGTAGT	TTGGCGGATTAGTTGGTGAG	20	20	55.0	50.0	58.2	57.9	5.0	0.0	0.324879	46091	47416	2143-2143	0.500000	57.4
chlN_p2	chlN	2	TGCTAGGCCAGAAGGAGTAG	TTGGCGGATTAGTTGGTGAG	20	20	55.0	50.0	58.2	57.9	5.0	0.0	0.326164	46091	47416	2144-2144	0.500000	57.4
chlN_p3	chlN	3	TTGCTAGGCCAGAAGGAGTA	TTGGCGGATTAGTTGGTGAG	20	20	50.0	50.0	57.8	57.9	5.0	0.0	0.355649	46091	47416	2145-2145	0.500000	57.2
chlN_p4	chlN	4	GATTGCTAGGCCAGAAGGAG	TTGGCGGATTAGTTGGTGAG	20	20	55.0	50.0	57.7	57.9	5.0	0.0	0.360926	46091	47416	2147-2147	0.500000	57.1
chlN_p5	chlN	5	GCTAGGCCAGAAGGAGTAGT	AAGTGGCGCTAGAGCAATTT	20	20	55.0	45.0	58.2	58.2	5.0	20.5	0.390544	46091	47416	1660-1660	0.500000	56.9
ycf37_p1	ycf37	1	TTTGAGCCTTCCAGTGTTCC	ACGGGATATAGCGCAGTTTG	20	20	50.0	50.0	58.0	58.1	0.0	11.5	0.152879	49365	49901	812-812	0.500000	58.8
ycf37_p2	ycf37	2	TTTGAGCCTTCCAGTGTTCC	ACAGTAGGCGAGAGTTCTGA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.220664	49365	49901	1361-1361	0.500000	58.2
ycf37_p3	ycf37	3	GCGTCCACTAGGTGTTTTCA	ACGGGATATAGCGCAGTTTG	20	20	50.0	50.0	58.1	58.1	0.0	11.5	0.260437	49365	49901	870-870	0.500000	57.9
ycf37_p4	ycf37	4	TTTGAGCCTTCCAGTGTTCC	GGGATATAGCGCAGTTTGGT	20	20	50.0	50.0	58.0	57.7	0.0	11.5	0.274861	49365	49901	810-810	0.500000	57.8
ycf37_p5	ycf37	5	AGCGTCCACTAGGTGTTTTC	ACGGGATATAGCGCAGTTTG	20	20	50.0	50.0	57.8	58.1	0.0	11.5	0.299454	49365	49901	871-871	0.500000	57.6
fabH_p1	fabH	1	AGTCAACTCAAGCCCGAAAG	ACAGCCCCAAGGATAAGACT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.143177	52511	53491	1584-1584	0.500000	58.9
fabH_p2	fabH	2	GCTGCAGGTTCACGAAAAAG	ACAGCCCCAAGGATAAGACT	20	20	50.0	50.0	58.2	58.0	17.1	0.0	0.268629	52511	53491	1904-1904	0.500000	57.9
fabH_p3	fabH	3	AACCTTGTCAGAACCGGAAG	ACAGCCCCAAGGATAAGACT	20	20	50.0	50.0	57.7	58.0	9.5	0.0	0.283530	52511	53491	1545-1545	0.500000	57.7
fabH_p4	fabH	4	AGTCAACTCAAGCCCGAAAG	ACTCGATAAGGACGGTGTCA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.285486	52511	53491	1555-1555	0.500000	57.7
fabH_p5	fabH	5	TTTACGCGATTATGGCTGGT	ACAGCCCCAAGGATAAGACT	20	20	45.0	50.0	57.7	58.0	15.9	0.0	0.346548	52511	53491	1875-1875	0.500000	57.2
rbcR_p1	rbcR	1	TTTGACTTCTGCCCGAAACA	CCAGTTAGTACGGACTTGGC	20	20	45.0	55.0	58.0	58.0	0.0	5.1	0.051532	59803	60756	2311-2311	0.500000	59.6
rbcR_p2	rbcR	2	TTTGACTTCTGCCCGAAACA	ATGCAGCTGCTAAAACTGGT	20	20	45.0	45.0	58.0	58.1	0.0	36.6	0.130420	59803	60756	1938-1938	0.500000	59.0
rbcR_p3	rbcR	3	CTTCTGCCCGAAACACATCT	CCAGTTAGTACGGACTTGGC	20	20	50.0	55.0	58.2	58.0	0.0	5.1	0.192457	59803	60756	2306-2306	0.500000	58.5
rbcR_p4	rbcR	4	CTTCTGCCCGAAACACATCT	ATGCAGCTGCTAAAACTGGT	20	20	50.0	45.0	58.2	58.1	0.0	36.6	0.271346	59803	60756	1933-1933	0.500000	57.8
rbcR_p5	rbcR	5	CCCTGAGAGCTAGGCCAATA	CCAGTTAGTACGGACTTGGC	20	20	55.0	55.0	58.3	58.0	5.0	5.1	0.283937	59803	60756	2190-2190	0.500000	57.7
rne_p1	rne	1	GGAGCTACAGTACAGGCAAC	TTCCAAGCTGAGCACAACAT	20	20	55.0	45.0	58.0	58.0	0.0	7.4	0.026067	63142	64677	2780-2780	0.500000	59.8
rne_p2	rne	2	GGAGCTACAGTACAGGCAAC	GCCTGTAGTTGGTATCTGGC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.056613	63142	64677	2502-2502	0.500000	59.5
rne_p3	rne	3	GGAGCTACAGTACAGGCAAC	TCCGGTGAATCCTTACCAGT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.064556	63142	64677	2303-2303	0.500000	59.5
rne_p4	rne	4	GGAGCTACAGTACAGGCAAC	CCCAATTGGCCAAGGTAGTT	20	20	55.0	50.0	58.0	58.1	0.0	24.9	0.074349	63142	64677	2853-2853	0.500000	59.4
rne_p5	rne	5	GGAGCTACAGTACAGGCAAC	CGTGAAACAAGCGAAAACGA	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.091481	63142	64677	2663-2663	0.500000	59.3
orf121_p1	orf121	1	TATCCCTAAGACACGCAGCA	ATTCCTGAACAGCCACCTTG	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.604070	67977	68342	1605-1605	0.500000	55.2
orf121_p2	orf121	2	TATCCCTAAGACACGCAGCA	CATTCCTGAACAGCCACCTT	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.604070	67977	68342	1606-1606	0.500000	55.2
orf121_p3	orf121	3	TCCCTAAGACACGCAGCATA	ATTCCTGAACAGCCACCTTG	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.604070	67977	68342	1603-1603	0.500000	55.2
orf121_p4	orf121	4	TCCCTAAGACACGCAGCATA	CATTCCTGAACAGCCACCTT	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.604070	67977	68342	1604-1604	0.500000	55.2
orf121_p5	orf121	5	CCCTAAGACACGCAGCATAT	ATTCCTGAACAGCCACCTTG	20	20	50.0	50.0	57.5	58.1	0.0	0.0	0.618125	67977	68342	1602-1602	0.500000	55.1
orf745_p1	orf745	1	GCCTCTGACTCTCCATTTGG	TCCGAGCAAAAAGATACTGCA	20	21	55.0	42.9	58.0	57.9	0.0	0.0	1.087985	80524	82668	2947-2947	0.500000	51.3
orf745_p2	orf745	2	GCCTCTGACTCTCCATTTGG	TCCACTTTCCTTTGCTACGA	20	20	55.0	45.0	58.0	56.8	0.0	0.0	1.248274	80524	82668	2556-2556	0.500000	50.0
orf745_p3	orf745	3	TTTAACGGCATTGGCACTCT	TCCACTTTCCTTTGCTACGA	20	20	45.0	45.0	58.1	56.8	11.1	0.0	1.308457	80524	82668	2899-2899	0.500000	49.5
orf745_p4	orf745	4	GGCATTGGCACTCTCATTTG	TCCACTTTCCTTTGCTACGA	20	20	50.0	45.0	57.7	56.8	5.2	0.0	1.501704	80524	82668	2893-2893	0.500000	48.0
orf745_p5	orf745	5	GCATTGGCACTCTCATTTGC	TCCACTTTCCTTTGCTACGA	20	20	50.0	45.0	58.4	56.8	0.0	0.0	1.575755	80524	82668	2892-2892	0.500000	47.4
orf286_p1	orf286	1	CATGACCGAAGTCGTCACAT	GACGCTTTAAATGTGCCCAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.026520	85006	85866	1380-1380	0.500000	59.8
orf286_p2	orf286	2	AAAGTTCCGGTTGCTCCAAT	GACGCTTTAAATGTGCCCAC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.027435	85006	85866	1497-1497	0.500000	59.8
orf286_p3	orf286	3	AAGTTCCGGTTGCTCCAATT	GACGCTTTAAATGTGCCCAC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.027435	85006	85866	1496-1496	0.500000	59.8
orf286_p4	orf286	4	GAACCCTCATGACCGAAGTC	GACGCTTTAAATGTGCCCAC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.034352	85006	85866	1387-1387	0.500000	59.7
orf286_p5	orf286	5	TGCGTATACCAATTTCGCCA	GACGCTTTAAATGTGCCCAC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.048400	85006	85866	1349-1349	0.500000	59.6
sufB_p1	sufB	1	TGTATTAAAGGGGATCGGCG	AGGCCGGAATCTGTTTCATC	20	20	50.0	50.0	57.5	58.0	0.0	5.0	0.580884	85976	87439	2651-2651	0.500000	55.4
sufB_p2	sufB	2	TGTATTAAAGGGGATCGGCG	GGCCGGAATCTGTTTCATCT	20	20	50.0	50.0	57.5	58.0	0.0	5.0	0.580884	85976	87439	2650-2650	0.500000	55.4
sufB_p3	sufB	3	GTATTAAAGGGGATCGGCGT	AGGCCGGAATCTGTTTCATC	20	20	50.0	50.0	57.5	58.0	6.8	5.0	0.582634	85976	87439	2650-2650	0.500000	55.3
sufB_p4	sufB	4	GTATTAAAGGGGATCGGCGT	GGCCGGAATCTGTTTCATCT	20	20	50.0	50.0	57.5	58.0	6.8	5.0	0.582634	85976	87439	2649-2649	0.500000	55.3
sufB_p5	sufB	5	TGTATTAAAGGGGATCGGCG	TCTAGATTTTGCGCAAGGCT	20	20	50.0	45.0	57.5	57.9	0.0	23.7	0.650621	85976	87439	2834-2834	0.500000	54.8
rps2_p1	rps2	1	CTGGTCGACGAGCTACAAAA	ACGGAAGCTGCTATTTCTGG	20	20	50.0	50.0	57.9	58.0	25.6	0.9	0.090164	93710	94435	1445-1445	0.500000	59.3
rps2_p2	rps2	2	CTGGTCGACGAGCTACAAAA	CGGAAGCTGCTATTTCTGGT	20	20	50.0	50.0	57.9	58.0	25.6	0.9	0.090164	93710	94435	1444-1444	0.500000	59.3
rps2_p3	rps2	3	CTGGTCGACGAGCTACAAAA	GCCGCAAGTTTTCAAGAGAC	20	20	50.0	50.0	57.9	58.0	25.6	0.0	0.098266	93710	94435	1478-1478	0.500000	59.2
rps2_p4	rps2	4	GCTTTTACAGTCTGAGCGGA	ACGGAAGCTGCTATTTCTGG	20	20	50.0	50.0	57.9	58.0	6.9	0.9	0.110124	93710	94435	1209-1209	0.500000	59.1
rps2_p5	rps2	5	GCTTTTACAGTCTGAGCGGA	CGGAAGCTGCTATTTCTGGT	20	20	50.0	50.0	57.9	58.0	6.9	0.9	0.110124	93710	94435	1208-1208	0.500000	59.1
atpB_p1	atpB	1	CCACGTCAACTATGCCAAGA	AATTGAGTGGCTGCTTCCAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.093658	108936	110363	2431-2431	0.500000	59.3
atpB_p2	atpB	2	CCACGTCAACTATGCCAAGA	ATTGAGTGGCTGCTTCCAAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.093658	108936	110363	2430-2430	0.500000	59.3
atpB_p3	atpB	3	CCACGTCAACTATGCCAAGA	TGAGTGGCTGCTTCCAAATT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.093658	108936	110363	2428-2428	0.500000	59.3
atpB_p4	atpB	4	CCACGTCAACTATGCCAAGA	TTGAGTGGCTGCTTCCAAAT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.093658	108936	110363	2429-2429	0.500000	59.3
atpB_p5	atpB	5	TCCACGTCAACTATGCCAAG	TGAGTGGCTGCTTCCAAATT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.093658	108936	110363	2429-2429	0.500000	59.3
tatC_p1	tatC	1	TTGAGGCAGTCCGATAAAGC	GGTAATACTGCCTGAGGTGC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.069249	115190	115969	1721-1721	0.500000	59.4
tatC_p2	tatC	2	TTGAGGCAGTCCGATAAAGC	GTAATACTGCCTGAGGTGCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.069249	115190	115969	1720-1720	0.500000	59.4
tatC_p3	tatC	3	TTGAGGCAGTCCGATAAAGC	GTGCCTCAATTTCTACCGGT	20	20	50.0	50.0	58.0	57.9	0.0	14.0	0.133600	115190	115969	1705-1705	0.500000	58.9
tatC_p4	tatC	4	CTTGCTGCTCTGCTTGAGTA	GGTAATACTGCCTGAGGTGC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.135473	115190	115969	1751-1751	0.500000	58.9
tatC_p5	tatC	5	CTTGCTGCTCTGCTTGAGTA	GTAATACTGCCTGAGGTGCC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.135473	115190	115969	1750-1750	0.500000	58.9
psaA_p1	psaA	1	TCCTTGTCTCAAGAGAGGAGA	AAGCTTGGCTAAACTTAGGA	21	20	47.6	40.0	57.2	54.2	10.5	0.0	5.570147	131183	133441	2346-2347	1.000000	48.0
psaA_p2	psaA	2	TCCTTGTCTCAAGAGAGGAGA	AGCTTGGCTAAACTTAGGAA	21	20	47.6	40.0	57.2	54.2	10.5	0.0	5.570147	131183	133441	2345-2346	1.000000	48.0
psaA_p3	psaA	3	GCGTTTTGATTCCTTGTCTCA	AAGCTTGGCTAAACTTAGGA	21	20	42.9	40.0	57.1	54.2	0.0	0.0	5.650397	131183	133441	2356-2357	1.000000	48.0
psaA_p4	psaA	4	GCGTTTTGATTCCTTGTCTCA	AGCTTGGCTAAACTTAGGAA	21	20	42.9	40.0	57.1	54.2	0.0	0.0	5.650397	131183	133441	2355-2356	1.000000	48.0
psaA_p5	psaA	5	TCCTTGTCTCAAGAGAGGAGA	AGCTTGGCTAAACTTAGGAAA	21	21	47.6	38.1	57.2	55.1	10.5	0.0	5.704260	131183	133441	2345-2346	1.000000	48.0
dnaB_p1	dnaB	1	GATCCTCGTGTCACCAGTTC	AAAACTGGAGACGGGGAAAC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.025275	170483	172192	2053-2053	0.500000	59.8
dnaB_p2	dnaB	2	TCAGTTGGTAGAGCAGTGGA	AAAACTGGAGACGGGGAAAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032625	170483	172192	2078-2078	0.500000	59.7
dnaB_p3	dnaB	3	GATCCTCGTGTCACCAGTTC	TGTTACAGTTGCAGCAGGTT	20	20	55.0	45.0	58.0	57.9	0.0	2.5	0.053665	170483	172192	2246-2246	0.500000	59.6
dnaB_p4	dnaB	4	GATCCTCGTGTCACCAGTTC	TTGTTACAGTTGCAGCAGGT	20	20	55.0	45.0	58.0	57.9	0.0	2.5	0.053665	170483	172192	2247-2247	0.500000	59.6
dnaB_p5	dnaB	5	TCAGTTGGTAGAGCAGTGGA	TGTTACAGTTGCAGCAGGTT	20	20	50.0	45.0	58.0	57.9	0.0	2.5	0.061016	170483	172192	2271-2271	0.500000	59.5
orf382b_p1	orf382b	1	TCTCTCTCCGTGTCGAGAAA	TTGTCCCACAGAAGGAGCTA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.204608	173446	174270	1273-1273	0.500000	58.4
orf382b_p2	orf382b	2	CGCCTGGTGTTATTCTGTGT	TTGTCCCACAGAAGGAGCTA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.215765	173446	174270	1539-1539	0.500000	58.3
orf382b_p3	orf382b	3	CTTGCTTTTGGTTTCGCACT	TTGTCCCACAGAAGGAGCTA	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.232502	173446	174270	1631-1631	0.500000	58.1
orf382b_p4	orf382b	4	GGGTTCGAATCCCTCTCTCT	TTGTCCCACAGAAGGAGCTA	20	20	55.0	50.0	58.2	58.0	17.2	0.0	0.241104	173446	174270	1286-1286	0.500000	58.1
orf382b_p5	orf382b	5	AATCCCTCTCTCTCCGTGTC	TTGTCCCACAGAAGGAGCTA	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.253881	173446	174270	1279-1279	0.500000	58.0
ntcA_p1	ntcA	1	TCAACCAGCCAAAGTTGACA	GCTGGTGATGGATTCTCTCG	20	20	45.0	55.0	57.9	58.1	1.6	0.0	0.273031	178350	178952	1527-1527	0.500000	57.8
ntcA_p2	ntcA	2	TTTCTCCAACAACAGGCACA	GCTGGTGATGGATTCTCTCG	20	20	45.0	55.0	57.9	58.1	0.0	0.0	0.273031	178350	178952	1773-1773	0.500000	57.8
ntcA_p3	ntcA	3	TCAACCAGCCAAAGTTGACA	GGCTGGTGATGGATTCTCTC	20	20	45.0	55.0	57.9	57.7	1.6	0.0	0.389275	178350	178952	1528-1528	0.500000	56.9
ntcA_p4	ntcA	4	TTTCTCCAACAACAGGCACA	GGCTGGTGATGGATTCTCTC	20	20	45.0	55.0	57.9	57.7	0.0	0.0	0.389275	178350	178952	1774-1774	0.500000	56.9
ntcA_p5	ntcA	5	TAAAATCACGCCGGTTACCC	GCTGGTGATGGATTCTCTCG	20	20	50.0	55.0	58.3	58.1	1.9	0.0	0.400121	178350	178952	1655-1655	0.500000	56.8
rps1-syfB_p1	rps1-syfB	1	TGCTGATACAGGTGTACCGA	GGTCTAAACCCGCACATTCT	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.267373	180951	181490	1298-1298	0.500000	57.9
rps1-syfB_p2	rps1-syfB	2	TGCTGATACAGGTGTACCGA	TGGTCTAAACCCGCACATTC	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.341874	180951	181490	1299-1299	0.500000	57.3
rps1-syfB_p3	rps1-syfB	3	TCTTCAGCGAGCAATTGTCT	GGTCTAAACCCGCACATTCT	20	20	45.0	50.0	57.5	57.9	7.4	0.0	0.571881	180951	181490	1483-1483	0.500000	55.4
rps1-syfB_p4	rps1-syfB	4	TGCTGATACAGGTGTACCGA	GTGGTCTAAACCCGCACATT	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.627625	180951	181490	1300-1300	0.500000	55.0
rps1-syfB_p5	rps1-syfB	5	TCTTCAGCGAGCAATTGTCT	TGGTCTAAACCCGCACATTC	20	20	45.0	50.0	57.5	58.2	7.4	0.0	0.646382	180951	181490	1484-1484	0.500000	54.8
syfB_p1	syfB	1	AACTGGTAAGGGTGCAAAGG	CGAGATGGTAGCACAGGAAC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.076729	181491	183587	2760-2760	0.500000	59.4
syfB_p2	syfB	2	GTGCCACAGAAATAAACCGC	CGAGATGGTAGCACAGGAAC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.094917	181491	183587	2796-2796	0.500000	59.2
syfB_p3	syfB	3	AACTGGTAAGGGTGCAAAGG	AAGGTCTCGAGATGGTAGCA	20	20	50.0	50.0	58.0	57.9	0.0	19.9	0.150021	181491	183587	2767-2767	0.500000	58.8
syfB_p4	syfB	4	AACTGGTAAGGGTGCAAAGG	TCGAGATGGTAGCACAGGAA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.157295	181491	183587	2761-2761	0.500000	58.7
syfB_p5	syfB	5	GTGCCACAGAAATAAACCGC	AAGGTCTCGAGATGGTAGCA	20	20	50.0	50.0	58.0	57.9	0.0	19.9	0.168209	181491	183587	2803-2803	0.500000	58.7