Hotspot

ycf3

LSC · 43925-45901 · 1977 bp

Back to Bambusa

Pi 0.00129

MarkerSeek score 46.90

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1977
variable_sites: 15
pi: 0.00129
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TCTTGATTCGATCTTCCGACC	TCGAATGTGTTTGTTTTTATGGT	47.6	30.4	57.6	55.6	2128-2135 bp	1.0	40.8
ycf3_p2	CTTGATTCGATCTTCCGACCT	TCGAATGTGTTTGTTTTTATGGT	47.6	30.4	57.6	55.6	2127-2134 bp	1.0	40.8
ycf3_p3	TTGATTCGATCTTCCGACCT	TCGAATGTGTTTGTTTTTATGGT	45.0	30.4	56.4	55.6	2126-2133 bp	1.0	40.8
ycf3_p4	TCTTGATTCGATCTTCCGACC	CGAATGTGTTTGTTTTTATGGT	47.6	31.8	57.6	54.2	2127-2134 bp	1.0	40.8
ycf3_p5	CTTGATTCGATCTTCCGACCT	CGAATGTGTTTGTTTTTATGGT	47.6	31.8	57.6	54.2	2126-2133 bp	1.0	40.8