Hotspot

rpoC2

LSC · 26729-31249 · 4521 bp

Back to Bambusa

Pi 0.00104

MarkerSeek score 37.20

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4521
variable_sites: 26
pi: 0.00104
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TTCGCTCCACAAGGATCAAG	TTGTTCTCTGGGCTCCCATA	50.0	50.0	57.9	58.0	519-720 bp	1.0	84.3
rpoC2_p2	AATCATGTCCAACGGCCATT	TTGTTCTCTGGGCTCCCATA	45.0	50.0	58.1	58.0	357-558 bp	1.0	84.1
rpoC2_p3	TTACCATGCGCCCGAATATC	TTGTTCTCTGGGCTCCCATA	50.0	50.0	58.2	58.0	624-825 bp	1.0	83.3
rpoC2_p4	TTCGCTCCACAAGGATCAAG	GGGCTCCCATATTCGTCTTC	50.0	55.0	57.9	57.8	585-786 bp	1.0	83.0
rpoC2_p5	GGGGAAATGCACTGGAGTAC	TTGTTCTCTGGGCTCCCATA	55.0	50.0	58.2	58.0	650-851 bp	1.0	82.9