Hotspot

rpl16

LSC · 79962-81463 · 1502 bp

Back to Bambusa

Pi 0.00193

MarkerSeek score 36.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1502
variable_sites: 21
pi: 0.00193
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CCAGAAAGAAAAACCTGGTT	AGCCTTATTTTTCCTGAAATCCC	40.0	39.1	53.9	57.1	1650-1688 bp	0.972973	39.6
rpl16_p2	CCAGAAAGAAAAACCTGGTT	GCCTTATTTTTCCTGAAATCCCT	40.0	39.1	53.9	57.1	1649-1687 bp	0.972973	39.6
rpl16_p3	CCAGAAAGAAAAACCTGGTT	TGACAAGAGCCTTATTTTTCCTG	40.0	39.1	53.9	57.0	1657-1695 bp	0.972973	39.6
rpl16_p4	CCAGAAAGAAAAACCTGGTT	TCCTGAAATCCCTGAAAAAGAAGA	40.0	37.5	53.9	58.0	1639-1677 bp	0.972973	39.6
rpl16_p5	CCAGAAAGAAAAACCTGGTTT	GCCTTATTTTTCCTGAAATCCCT	38.1	39.1	54.8	57.1	1649-1687 bp	0.972973	39.6