Hotspot

rpl14-rpl16

LSC · 79853-79961 · 109 bp

Back to Bambusa

Pi 0.00741
MarkerSeek score 47.90
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
109
variable_sites
8
pi
0.00741
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 AGCAGCTCCTATGACTCGAA TAGCTGCCAGTAAAATGCCC 50.0 50.0 58.2 58.2 252-269 bp 1.0 79.7
rpl14-rpl16_p2 CGAGCTCCACTGTTATCTGC TAGCTGCCAGTAAAATGCCC 55.0 50.0 58.4 58.2 218-235 bp 1.0 78.7
rpl14-rpl16_p3 TAGCAGCTCCTATGACTCGA TAGCTGCCAGTAAAATGCCC 50.0 50.0 57.4 58.2 253-270 bp 1.0 76.5
rpl14-rpl16_p4 AGCAGCTCCTATGACTCGAA GCCAGTAAAATGCCCATACG 50.0 50.0 58.2 57.2 247-264 bp 1.0 75.5
rpl14-rpl16_p5 GCAGCTCCTATGACTCGAAT TAGCTGCCAGTAAAATGCCC 50.0 50.0 57.2 58.2 251-268 bp 1.0 75.1