Hotspot

rbcL-psaI

LSC · 58045-59289 · 1245 bp

Back to Bambusa

Pi 0.00317

MarkerSeek score 48.60

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1245
variable_sites: 24
pi: 0.00317
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	CGCAGCTTGTGAAGTATGGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.3	58.0	1376-1385 bp	1.0	79.4
rbcL-psaI_p2	CGCAGCTTGTGAAGTATGGA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.3	57.9	1382-1391 bp	1.0	78.8
rbcL-psaI_p3	GGCGATCAAATTCGAGTTCG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.6	58.0	1355-1364 bp	1.0	78.8
rbcL-psaI_p4	TGGAAGGCGATCAAATTCGA	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.6	58.0	1360-1369 bp	1.0	78.5
rbcL-psaI_p5	GGCGATCAAATTCGAGTTCG	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.6	57.9	1361-1370 bp	1.0	78.2