Hotspot

ycf1

SSC · 121849-127404 · 5556 bp

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Pi 0.00054
MarkerSeek score 52.80
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5556
variable_sites
3
pi
0.00054
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 ACTCGAAATAGACGGAATTGGA TCTTAGTCCTACAAGTTATAATGCT 40.9 32.0 57.3 55.3 289 bp 1.0 40.3
ycf1_p2 TCGAAATAGACGGAATTGGAGT TCTTAGTCCTACAAGTTATAATGCT 40.9 32.0 57.3 55.3 287 bp 1.0 40.3
ycf1_p3 TCGAAATAGACGGAATTGGAGTT TCTTAGTCCTACAAGTTATAATGCT 39.1 32.0 57.9 55.3 287 bp 1.0 40.3
ycf1_p4 ACTCGAAATAGACGGAATTGGA TCTTAGTCCTACAAGTTATAATGC 40.9 33.3 57.3 54.0 289 bp 1.0 40.3
ycf1_p5 TCGAAATAGACGGAATTGGAGT TCTTAGTCCTACAAGTTATAATGC 40.9 33.3 57.3 54.0 287 bp 1.0 40.3