Hotspot

rps4

LSC · 44864-45469 · 606 bp

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Pi 0

MarkerSeek score 28.30

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: LSC
length_bp: 606
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	AAGAAAATTGCCCGAACCCT	TGAAAAAGTGCCTTCCCCTC	45.0	50.0	58.0	58.0	975-979 bp	1.0	80.0
rps4_p2	AGAAAATTGCCCGAACCCTT	TGAAAAAGTGCCTTCCCCTC	45.0	50.0	58.0	58.0	974-978 bp	1.0	80.0
rps4_p3	AAGAAAATTGCCCGAACCCT	GCCTTCCCCTCTTTCCAAAA	45.0	50.0	58.0	58.0	966-970 bp	1.0	80.0
rps4_p4	AGAAAATTGCCCGAACCCTT	GCCTTCCCCTCTTTCCAAAA	45.0	50.0	58.0	58.0	965-969 bp	1.0	80.0
rps4_p5	AGAAAATTGCCCGAACCCTT	TTTCTATTATGTGGGCCCGC	45.0	50.0	58.0	58.0	1136-1140 bp	1.0	79.9