Hotspot

rps19

LSC · 82837-83115 · 279 bp

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Pi 0.00717
MarkerSeek score 41.80
Primer available yes
Label rps19

Feature Metadata

label_name
rps19
feature_type
gene
region
LSC
length_bp
279
variable_sites
2
pi
0.00717
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19_p1 GGCCCCGAATCTGATCAATT AGATATCCACCCATGCGGTA 50.0 50.0 58.0 58.0 1068 bp 1.0 79.9
rps19_p2 CGCATAACTACGTCCTCGAG AGATATCCACCCATGCGGTA 55.0 50.0 58.0 58.0 1274 bp 1.0 79.8
rps19_p3 CAAGTCGGAAACCAAGTGGA AGATATCCACCCATGCGGTA 50.0 50.0 58.0 58.0 1457 bp 1.0 79.7
rps19_p4 GCTTCGTCGGAACCCATATT AGATATCCACCCATGCGGTA 50.0 50.0 58.1 58.0 1189 bp 1.0 79.6
rps19_p5 GATGGCTTTGGGTTGTACCA AGATATCCACCCATGCGGTA 50.0 50.0 58.1 58.0 1475 bp 1.0 79.3