Hotspot

rpl36

LSC · 78581-78694 · 114 bp

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Pi 0.0175
MarkerSeek score 62.60
Primer available yes
Label rpl36

Feature Metadata

label_name
rpl36
feature_type
gene
region
LSC
length_bp
114
variable_sites
2
pi
0.0175
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36_p1 ACGTTTAGTCCTTTGGTGAGT AGGATTCGAAAGATTAGGTGGT 42.9 40.9 57.2 56.9 252 bp 1.0 41.1
rpl36_p2 CGTTTAGTCCTTTGGTGAGTCT AGGATTCGAAAGATTAGGTGGT 45.5 40.9 58.0 56.9 251 bp 1.0 40.6
rpl36_p3 ACGTTTAGTCCTTTGGTGAGT AAGGATTCGAAAGATTAGGTGGT 42.9 39.1 57.2 57.6 253 bp 1.0 40.6
rpl36_p4 ACGTTTAGTCCTTTGGTGAGT AGGATTCGAAAGATTAGGTGGTT 42.9 39.1 57.2 57.6 252 bp 1.0 40.6
rpl36_p5 ACGTTTAGTCCTTTGGTGAGTC AGGATTCGAAAGATTAGGTGGT 45.5 40.9 58.3 56.9 252 bp 1.0 40.6