Hotspot

petD

LSC · 75593-76800 · 1208 bp

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Pi 0.00166

MarkerSeek score 59.70

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1208
variable_sites: 2
pi: 0.00166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	AGTTGAAGGGAATTCTCCGA	TCCCTAGATACACACGTCGT	45.0	50.0	56.2	57.6	1284 bp	1.0	62.1
petD_p2	TGAAGGGAATTCTCCGAAGAG	TCCCTAGATACACACGTCGT	47.6	50.0	57.1	57.6	1281 bp	1.0	61.7
petD_p3	AGTTGAAGGGAATTCTCCGAAG	TCCCTAGATACACACGTCGT	45.5	50.0	58.1	57.6	1284 bp	1.0	60.4
petD_p4	GTTGAAGGGAATTCTCCGAAGA	TCCCTAGATACACACGTCGT	45.5	50.0	58.1	57.6	1283 bp	1.0	60.4
petD_p5	AGTTGAAGGGAATTCTCCGAA	TCCCTAGATACACACGTCGT	42.9	50.0	56.9	57.6	1284 bp	1.0	60.3