Hotspot

rpl32-trnL-UAG

SSC · 111549-112274 · 726 bp

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Pi 0.00275
MarkerSeek score 60.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
726
variable_sites
2
pi
0.00275
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGTATCAGGCAGCGTTAAA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 863 bp 1.0 79.9
rpl32-trnL-UAG_p2 GGGTATCAGGCAGCGTTAAA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 864 bp 1.0 79.9
rpl32-trnL-UAG_p3 GGGTATCAGGCAGCGTTAAA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 873 bp 1.0 78.9
rpl32-trnL-UAG_p4 GGGTATCAGGCAGCGTTAAA AAGAGATGATATGCCGCCAC 50.0 50.0 58.0 57.8 925 bp 1.0 78.5
rpl32-trnL-UAG_p5 GGGTATCAGGCAGCGTTAAA CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.0 57.8 871 bp 1.0 78.4