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rpl22-rps19

LSC · 82779-82836 · 58 bp

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Pi 0.0172

MarkerSeek score 60.50

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 58
variable_sites: 1
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	GGCCCCGAATCTGATCAATT	TGAATTTCCAAGGACACGCA	50.0	45.0	58.0	58.0	202 bp	1.0	80.1
rpl22-rps19_p2	TGTCGACCCAAAGCGTATAC	TGAATTTCCAAGGACACGCA	50.0	45.0	57.7	58.0	149 bp	1.0	78.1
rpl22-rps19_p3	GTCGACCCAAAGCGTATACT	TGAATTTCCAAGGACACGCA	50.0	45.0	57.4	58.0	148 bp	1.0	75.7
rpl22-rps19_p4	GGCCCCGAATCTGATCAATT	CGATAATAGATCCCGCCGTT	50.0	50.0	58.0	57.4	177 bp	1.0	75.2
rpl22-rps19_p5	GGCCCCGAATCTGATCAATT	AAGCGATAATAGATCCCGCC	50.0	50.0	58.0	57.3	180 bp	1.0	74.9