Hotspot

ndhC-trnV-UAC

LSC · 49920-50882 · 963 bp

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Pi 0.00104
MarkerSeek score 58.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
963
variable_sites
1
pi
0.00104
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TTCTCCGGCCCTTTCCTAAT TGCATGTTGGGTCCTTGAAA 50.0 45.0 58.4 57.9 1179-1180 bp 1.0 76.2
ndhC-trnV-UAC_p2 TTCTCCGGCCCTTTCCTAAT CCGAGAAAGTCTACGGTTCG 50.0 55.0 58.4 58.1 1117-1118 bp 1.0 76.0
ndhC-trnV-UAC_p3 CCCTTTCCTAATCGGGGCTA TGCATGTTGGGTCCTTGAAA 55.0 45.0 58.6 57.9 1171-1172 bp 1.0 75.0
ndhC-trnV-UAC_p4 CCCTTTCCTAATCGGGGCTA CCGAGAAAGTCTACGGTTCG 55.0 55.0 58.6 58.1 1109-1110 bp 1.0 74.7
ndhC-trnV-UAC_p5 TTTCTCCGGCCCTTTCCTAA TGCATGTTGGGTCCTTGAAA 50.0 45.0 58.6 57.9 1180-1181 bp 1.0 74.4