Hotspot

rps14-psaB

LSC · 36927-37053 · 127 bp

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Pi 0.0157

MarkerSeek score 61.60

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 127
variable_sites: 2
pi: 0.0157
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	TGCCTCTTCTTCTCCCTTTG	CCTTTCCATTGTGCAAGCAA	50.0	45.0	57.1	57.5	277 bp	1.0	69.4
rps14-psaB_p2	TTGCCTCTTCTTCTCCCTTTG	CCTTTCCATTGTGCAAGCAA	47.6	45.0	57.9	57.5	278 bp	1.0	67.2
rps14-psaB_p3	GCCTCTTCTTCTCCCTTTGA	CCTTTCCATTGTGCAAGCAA	50.0	45.0	56.8	57.5	276 bp	1.0	67.1
rps14-psaB_p4	TGCCTCTTCTTCTCCCTTTG	CATTGTGCAAGCAAGATTGGT	50.0	42.9	57.1	58.0	271 bp	1.0	65.4
rps14-psaB_p5	TGCCTCTTCTTCTCCCTTTG	CTGTGGCCCTTTCCATTGT	50.0	52.6	57.1	57.9	284 bp	1.0	65.2