Hotspot

trnK-UUU-rps16

LSC · 4194-4957 · 764 bp

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Pi 0.00131
MarkerSeek score 59.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
764
variable_sites
1
pi
0.00131
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TTTCCTTGAAAGGGGTGCTC 50.0 50.0 58.2 58.0 901 bp 1.0 78.7
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TTTCCTTGAAAGGGGTGCTC 50.0 50.0 58.2 58.0 900 bp 1.0 78.7
trnK-UUU-rps16_p3 TCTAGCCGCACTTAAAAGCC TTTCCTTGAAAGGGGTGCTC 50.0 50.0 58.3 58.0 915 bp 1.0 77.9
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA TTTCCTTGAAAGGGGTGCTC 50.0 50.0 58.3 58.0 910 bp 1.0 77.8
trnK-UUU-rps16_p5 TACCGTTGAGTTAGCAACCC TTTCCTTGAAAGGGGTGCTC 50.0 50.0 57.5 58.0 887 bp 1.0 76.4