Hotspot

ycf1

SSC · 129742-135447 · 5706 bp

Back to Balanops

Pi 0.00597

MarkerSeek score 41.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5706
variable_sites: 34
pi: 0.00597
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACTTGATTTTCCAGCAAATTCA	GGGATCTCTTGCTCGGGATA	31.8	55.0	55.1	58.4	328 bp	1.0	41.7
ycf1_p2	ACTTGATTTTCCAGCAAATTCA	TAAGGGATCTCTTGCTCGGG	31.8	55.0	55.1	58.6	331 bp	1.0	41.7
ycf1_p3	AACTTGATTTTCCAGCAAATTCA	GGGATCTCTTGCTCGGGATA	30.4	55.0	55.8	58.4	329 bp	1.0	41.7
ycf1_p4	ACTTGATTTTCCAGCAAATTCAT	GGGATCTCTTGCTCGGGATA	30.4	55.0	55.6	58.4	328 bp	1.0	41.7
ycf1_p5	AACTTGATTTTCCAGCAAATTCA	TAAGGGATCTCTTGCTCGGG	30.4	55.0	55.8	58.6	332 bp	1.0	41.7