Hotspot

ndhF

SSC · 115585-117837 · 2253 bp

Back to Balanops

Pi 0.00672

MarkerSeek score 47.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2253
variable_sites: 15
pi: 0.00672
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	TGGTTTTATTGGCTGACCCC	50.0	50.0	58.0	58.1	2791-2807 bp	1.0	80.6
ndhF_p2	GCAGAATACCGTCACCCATT	TGGTTTTATTGGCTGACCCC	50.0	50.0	58.0	58.1	2901-2917 bp	1.0	80.4
ndhF_p3	GACTAAACAGGAGCAAGGGG	TGGTTTTATTGGCTGACCCC	55.0	50.0	57.9	58.1	2783-2799 bp	1.0	79.8
ndhF_p4	GGACTAAACAGGAGCAAGGG	TGGTTTTATTGGCTGACCCC	55.0	50.0	57.9	58.1	2784-2800 bp	1.0	79.8
ndhF_p5	GGGACTAAACAGGAGCAAGG	TGGTTTTATTGGCTGACCCC	55.0	50.0	57.9	58.1	2785-2801 bp	1.0	79.8