Hotspot

ndhF-rpl32

SSC · 117838-118660 · 823 bp

Back to Balanops

Pi 0.00613

MarkerSeek score 63.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 823
variable_sites: 5
pi: 0.00613
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CGGAAAGAGTAGGAGTCCCA	CGCTGTCCAATATCCCTTCC	55.0	55.0	58.2	58.1	997-1037 bp	1.0	78.9
ndhF-rpl32_p2	TTTTTGTTGACGCCGGAAAG	CGCTGTCCAATATCCCTTCC	45.0	55.0	57.8	58.1	1010-1050 bp	1.0	78.5
ndhF-rpl32_p3	CGGAAAGAGTAGGAGTCCCA	GCTGTCCAATATCCCTTCCC	55.0	55.0	58.2	57.7	996-1036 bp	1.0	77.6
ndhF-rpl32_p4	TTTTTGTTGACGCCGGAAAG	GCTGTCCAATATCCCTTCCC	45.0	55.0	57.8	57.7	1009-1049 bp	1.0	77.2
ndhF-rpl32_p5	CGGAAAGAGTAGGAGTCCCA	TCAACGCTGTCCAATATCCC	55.0	50.0	58.2	57.7	1001-1041 bp	1.0	77.2