Hotspot

psbE-petL

LSC · 68865-70276 · 1412 bp

Back to Balanops

Pi 0.00948

MarkerSeek score 54.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1412
variable_sites: 13
pi: 0.00948
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	ACCGAAATAACTAGTTATAGTAGGCA	50.0	34.6	57.8	57.5	1437-1466 bp	1.0	43.0
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	ACCGAAATAACTAGTTATAGTAGGCA	55.0	34.6	58.2	57.5	1432-1461 bp	1.0	43.0
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	ACCGAAATAACTAGTTATAGTAGGCA	50.0	34.6	58.4	57.5	1430-1459 bp	1.0	43.0
psbE-petL_p4	TTCTCCTGTGCTTCCAGACA	ACCGAAATAACTAGTTATAGTAGGCA	50.0	34.6	58.6	57.5	1431-1460 bp	1.0	43.0
psbE-petL_p5	AAGAACGTTCTCCTGTGCTT	ACCGAAATAACTAGTTATAGTAGGCA	45.0	34.6	57.4	57.5	1438-1467 bp	1.0	43.0