Hotspot

ndhC-trnV-UAC

LSC · 53506-55046 · 1541 bp

Back to Balanops

Pi 0.00454
MarkerSeek score 63.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1541
variable_sites
6
pi
0.00454
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.86
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTCTGGCCCTTTGCTAATCG GCATGTTGGGTCTTTGAAGC 55.0 50.0 58.7 57.9 1575-1755 bp 1.0 78.2
ndhC-trnV-UAC_p2 TCTCTGGCCCTTTGCTAATC GCATGTTGGGTCTTTGAAGC 50.0 50.0 57.3 57.9 1576-1756 bp 1.0 78.0
ndhC-trnV-UAC_p3 CTCTGGCCCTTTGCTAATCG GGGTCTTTGAAGCAGTTCGA 55.0 50.0 58.7 58.1 1568-1748 bp 1.0 77.7
ndhC-trnV-UAC_p4 TCTCTGGCCCTTTGCTAATC GGGTCTTTGAAGCAGTTCGA 50.0 50.0 57.3 58.1 1569-1749 bp 1.0 77.6
ndhC-trnV-UAC_p5 CTCTGGCCCTTTGCTAATCG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.7 57.8 1511-1691 bp 1.0 77.1