Hotspot

trnN-GUU-ndhF

IRb · 112660-114091 · 1432 bp

Back to Bakerantha

Pi 0.00333

MarkerSeek score 62.90

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1432
variable_sites: 6
pi: 0.00333
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGTCAGTTAACAGCCAACCG	TATGGGAGGTGGACGAATCT	50.0	50.0	58.1	57.5	1566 bp	0.333333	48.6
trnN-GUU-ndhF_p2	ACGACCAGTCAGTTAACAGC	TATGGGAGGTGGACGAATCT	50.0	50.0	57.8	57.5	1572 bp	0.333333	48.4
trnN-GUU-ndhF_p3	AGGATTCGAACCTACGACCA	TATGGGAGGTGGACGAATCT	50.0	50.0	58.2	57.5	1585 bp	0.333333	48.3
trnN-GUU-ndhF_p4	GGATTCGAACCTACGACCAG	TATGGGAGGTGGACGAATCT	55.0	50.0	57.8	57.5	1584 bp	0.333333	47.9
trnN-GUU-ndhF_p5	CCCCAAGTAGGATTCGAACC	TATGGGAGGTGGACGAATCT	55.0	50.0	57.7	57.5	1593 bp	0.333333	46.9