Hotspot

petA-psbJ

LSC · 65568-66701 · 1134 bp

Back to Bakerantha

Pi 0.0239

MarkerSeek score 62.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1134
variable_sites: 40
pi: 0.0239
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCAATCCCAATGTAGGAGGG	TCTGGGTTGGGTTCATCTCT	55.0	50.0	58.0	58.0	1311-1332 bp	1.0	83.6
petA-psbJ_p2	GCAATCCCAATGTAGGAGGG	TGGGTTGGGTTCATCTCTCT	55.0	50.0	58.0	58.0	1309-1330 bp	1.0	83.6
petA-psbJ_p3	CAGGGCCAGAACTTCTTGTT	TCTGGGTTGGGTTCATCTCT	50.0	50.0	58.0	58.0	1371-1392 bp	1.0	83.5
petA-psbJ_p4	CAGGGCCAGAACTTCTTGTT	TGGGTTGGGTTCATCTCTCT	50.0	50.0	58.0	58.0	1369-1390 bp	1.0	83.5
petA-psbJ_p5	GCAATCCCAATGTAGGAGGG	GGACTAGCTGTACCTACCGT	55.0	55.0	58.0	58.0	1740-1761 bp	1.0	82.6