Hotspot

ndhC-trnV-UAC

LSC · 52528-53570 · 1043 bp

Back to Bakerantha

Pi 0.0211
MarkerSeek score 71.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1043
variable_sites
32
pi
0.0211
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTAACCGGGGCTAAAACTCC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1178-1183 bp 1.0 81.8
ndhC-trnV-UAC_p2 CGGGGCTAAAACTCCTGAAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 1173-1178 bp 1.0 80.5
ndhC-trnV-UAC_p3 TAACCGGGGCTAAAACTCCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.3 57.8 1177-1182 bp 1.0 79.4
ndhC-trnV-UAC_p4 CTAACCGGGGCTAAAACTCC GAAGGTCTACGGTTCGAGTC 55.0 55.0 58.0 57.5 1177-1182 bp 1.0 79.1
ndhC-trnV-UAC_p5 TCTCGGGTCCTTCACTAACC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.4 57.8 1192-1197 bp 1.0 78.5