Hotspot

trnT-GGU-psbD

LSC · 33710-34761 · 1052 bp

Back to Bakerantha

Pi 0.00982

MarkerSeek score 62.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1052
variable_sites: 15
pi: 0.00982
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1211-1215 bp	1.0	79.6
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1214-1218 bp	1.0	79.5
trnT-GGU-psbD_p3	ACGGCCCTTTTAACTCAGTG	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1228-1232 bp	1.0	79.3
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1201-1205 bp	1.0	78.6
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1204-1208 bp	1.0	78.4