Hotspot

rpoB-trnC-GCA

LSC · 27872-29188 · 1317 bp

Back to Bakerantha

Pi 0.0142

MarkerSeek score 62.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1317
variable_sites: 27
pi: 0.0142
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCCTCATTTCCATTCCGGA	AAAGGATTTGCAGTCCCCTG	50.0	50.0	58.4	58.1	1420-1452 bp	1.0	80.2
rpoB-trnC-GCA_p2	TCCCTCATTTCCATTCCGGA	GCGACACCCAGATTTGAACT	50.0	50.0	58.4	58.5	1446-1478 bp	1.0	76.9
rpoB-trnC-GCA_p3	TCCCTCATTTCCATTCCGGA	TTTGTTGATCAGGCGACACC	50.0	50.0	58.4	58.8	1458-1490 bp	1.0	74.5
rpoB-trnC-GCA_p4	TCCCTCATTTCCATTCCGGA	TTTTGTTGATCAGGCGACAC	50.0	45.0	58.4	56.9	1459-1491 bp	1.0	72.2
rpoB-trnC-GCA_p5	TCCCTCATTTCCATTCCGGA	GGCGACACCCAGATTTGAA	50.0	52.6	58.4	58.1	1447-1479 bp	1.0	72.2