Hotspot

atpH-atpI

LSC · 14881-15553 · 673 bp

Back to Bakerantha

Pi 0.0188

MarkerSeek score 64.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 673
variable_sites: 19
pi: 0.0188
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	730-1043 bp	1.0	80.1
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	786-1099 bp	1.0	79.4
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	CGAATCCATGGAGGGTCATC	45.0	55.0	57.9	57.8	746-1059 bp	1.0	79.2
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	731-1044 bp	1.0	78.9
atpH-atpI_p5	GAAGCAAGTCCTACAGCCAA	CGAATCCATGGAGGGTCATC	50.0	55.0	57.8	57.8	760-1073 bp	1.0	78.9