Hotspot

trnS-GCU-trnG-UCC

LSC · 8726-10106 · 1381 bp

Back to Bakerantha

Pi 0.016

MarkerSeek score 65.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1381
variable_sites: 27
pi: 0.016
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	GAGAAAGCGGAAAGAGAGGG	AACCGATGGACCCCTTAACT	55.0	50.0	58.0	58.3	948-1548 bp	1.0	82.8
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	AACCGATGGACCCCTTAACT	45.0	50.0	58.0	58.3	893-1493 bp	1.0	82.7
trnS-GCU-trnG-UCC_p3	GAGAAAGCGGAAAGAGAGGG	AAACCGATGGACCCCTTAAC	55.0	50.0	58.0	57.5	949-1549 bp	1.0	81.5
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	AAACCGATGGACCCCTTAAC	45.0	50.0	58.0	57.5	894-1494 bp	1.0	81.4
trnS-GCU-trnG-UCC_p5	GAGAAAGCGGAAAGAGAGGG	ACCGATGGACCCCTTAACTA	55.0	50.0	58.0	57.4	947-1547 bp	1.0	81.0