Hotspot

trnQ-UUG-psbK

LSC · 7448-7791 · 344 bp

Back to Bakerantha

Pi 0.0155

MarkerSeek score 63.30

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 344
variable_sites: 8
pi: 0.0155
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	ATGAAAACCGATCTGGGACG	GGGCAATTTGGCGAAGAAAA	50.0	45.0	58.0	57.8	509 bp	1.0	79.8
trnQ-UUG-psbK_p2	GGAATGCAGTGTGATCCCAT	GGGCAATTTGGCGAAGAAAA	50.0	45.0	57.9	57.8	539 bp	1.0	79.4
trnQ-UUG-psbK_p3	AGTGTGATCCCATCTCTCGT	GGGCAATTTGGCGAAGAAAA	50.0	45.0	57.8	57.8	532 bp	1.0	78.7
trnQ-UUG-psbK_p4	TGTGGAATGCAGTGTGATCC	GGGCAATTTGGCGAAGAAAA	50.0	45.0	58.2	57.8	542 bp	1.0	78.5
trnQ-UUG-psbK_p5	GCAGTGTGATCCCATCTCTC	GGGCAATTTGGCGAAGAAAA	55.0	45.0	57.8	57.8	534 bp	1.0	78.0