Hotspot

trnK-UUU-rps16

LSC · 4103-4807 · 705 bp

Back to Bakerantha

Pi 0.00762
MarkerSeek score 62.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
705
variable_sites
8
pi
0.00762
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 809-814 bp 1.0 78.4
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 808-813 bp 1.0 78.4
trnK-UUU-rps16_p3 TACCGTTGAGTTAGCAACCC GGTGCTCAACCTACAGGAAC 50.0 55.0 57.5 58.2 795-800 bp 1.0 76.1
trnK-UUU-rps16_p4 AGTCGCACTTAAAAGCCGAG GGTGCTCAACCTACAGGAAC 50.0 55.0 58.6 58.2 820-825 bp 1.0 75.2
trnK-UUU-rps16_p5 TCGCACTTAAAAGCCGAGTA GGTGCTCAACCTACAGGAAC 45.0 55.0 57.3 58.2 818-823 bp 1.0 73.9