Hotspot

rpl22

LSC · 83762-84157 · 396 bp

Back to Bactris

Pi 0.00126

MarkerSeek score 27.70

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 396
variable_sites: 1
pi: 0.00126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	AAGGAACGATGACTTTGGGT	TTAGTGGGGGATGACCTT	45.0	50.0	57.0	54.1	528 bp	1.0	40.2
rpl22_p2	AGGAACGATGACTTTGGGTT	TTAGTGGGGGATGACCTT	45.0	50.0	57.0	54.1	527 bp	1.0	40.2
rpl22_p3	GGAACGATGACTTTGGGTTG	TTAGTGGGGGATGACCTT	50.0	50.0	57.0	54.1	526 bp	1.0	40.2
rpl22_p4	CCAAGTCTGAAACCAAGTGGA	TTAGTGGGGGATGACCTT	47.6	50.0	58.1	54.1	504 bp	1.0	40.2
rpl22_p5	AAGGAACGATGACTTTGGGTT	TTAGTGGGGGATGACCTT	42.9	50.0	57.8	54.1	528 bp	1.0	40.2