Hotspot

psbE-petL

LSC · 64711-65621 · 911 bp

Back to Bactris

Pi 0.00549

MarkerSeek score 47.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 911
variable_sites: 10
pi: 0.00549
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	1057-1058 bp	1.0	76.6
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	1055-1056 bp	1.0	76.6
psbE-petL_p3	ACTGACGAATAACCAACCCG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	1070-1071 bp	1.0	76.3
psbE-petL_p4	ACTGACGAATAACCAACCCG	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	1068-1069 bp	1.0	76.3
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AAGCAGCCAGTAGAAAACCG	45.0	50.0	58.2	58.5	1066-1067 bp	1.0	75.6