Hotspot

petA-psbJ

LSC · 62924-63940 · 1017 bp

Back to Bactris

Pi 0.0118

MarkerSeek score 58.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1017
variable_sites: 24
pi: 0.0118
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.0	58.3	1164-1169 bp	1.0	79.2
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.0	57.6	1160-1165 bp	1.0	78.3
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	GGTTCATATTCCGGGTTGGG	50.0	55.0	57.8	58.3	1147-1152 bp	1.0	78.3
petA-psbJ_p4	ATCCATTACGTGTCCAAGGC	GTTCATATTCCGGGTTGGGT	50.0	50.0	58.0	57.6	1163-1168 bp	1.0	78.2
petA-psbJ_p5	CATTACGTGTCCAAGGCCTT	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.2	58.3	1161-1166 bp	1.0	78.1