Hotspot

ndhC-trnV-UAC

LSC · 49839-51753 · 1915 bp

Back to Bactris

Pi 0.000783
MarkerSeek score 51.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1915
variable_sites
3
pi
0.000783
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCTTCACTAACCGGTGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.1 57.8 2028-2060 bp 1.0 79.3
ndhC-trnV-UAC_p2 TAACCGGTGCTAAAACTCCG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 2020-2052 bp 1.0 79.2
ndhC-trnV-UAC_p3 TCCTTCACTAACCGGTGCTA AGCCCAATGCAACAAAATGC 50.0 45.0 58.1 58.5 2110-2142 bp 1.0 77.0
ndhC-trnV-UAC_p4 TAACCGGTGCTAAAACTCCG AGCCCAATGCAACAAAATGC 50.0 45.0 57.9 58.5 2102-2134 bp 1.0 76.9
ndhC-trnV-UAC_p5 TCCTTCACTAACCGGTGCTA GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.1 57.5 2027-2059 bp 1.0 76.5