Hotspot

trnT-GGU-psbD

LSC · 30878-31879 · 1002 bp

Back to Bactris

Pi 0.002

MarkerSeek score 50.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1002
variable_sites: 4
pi: 0.002
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AACGGGCCCTTTTAACTCAG	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1170-1178 bp	1.0	77.6
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1165-1173 bp	1.0	77.5
trnT-GGU-psbD_p3	AACGGGCCCTTTTAACTCAG	CCCTCCGTAACCAATCATCC	50.0	55.0	58.1	57.7	1154-1162 bp	1.0	77.5
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	CCCTCCGTAACCAATCATCC	50.0	55.0	57.9	57.7	1149-1157 bp	1.0	77.5
trnT-GGU-psbD_p5	AACGGGCCCTTTTAACTCAG	GCCAGACCAGCCTACAAAAA	50.0	50.0	58.1	58.4	1183-1191 bp	1.0	76.5