Hotspot

atpI-rps2

LSC · 14788-15046 · 259 bp

Back to Bactris

Pi 0.00644

MarkerSeek score 57.70

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 259
variable_sites: 3
pi: 0.00644
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	GTTGGCCTACTTCTACACCC	TTCCAGCAAATGATGACGCT	55.0	45.0	57.6	58.2	432-433 bp	1.0	76.3
atpI-rps2_p2	GTTGGCCTACTTCTACACCC	TGCAATTTGTGAGGGTCGTT	55.0	45.0	57.6	58.3	374-375 bp	1.0	75.4
atpI-rps2_p3	GTTGGCCTACTTCTACACCC	GCAATTTGTGAGGGTCGTTC	55.0	50.0	57.6	57.7	373-374 bp	1.0	75.2
atpI-rps2_p4	GTTGGCCTACTTCTACACCC	TTGTGAGGGTCGTTCTAGCT	55.0	50.0	57.6	58.4	368-369 bp	1.0	74.9
atpI-rps2_p5	GGCCTACTTCTACACCCGAT	TTCCAGCAAATGATGACGCT	55.0	45.0	58.6	58.2	429-430 bp	1.0	74.8