Hotspot

trnS-GCU-trnG-UCC

LSC · 7547-8368 · 822 bp

Back to Bactris

Pi 0.00365

MarkerSeek score 47.80

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 822
variable_sites: 6
pi: 0.00365
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	AACGGAAAGACCCCTTAACT	45.0	45.0	58.0	56.4	934-953 bp	1.0	67.5
trnS-GCU-trnG-UCC_p2	AGAAGAAGCGGAGAGAGAGG	AACGGAAAGACCCCTTAACT	55.0	45.0	58.2	56.4	990-1009 bp	1.0	65.8
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	AAACGGAAAGACCCCTTAACT	45.0	42.9	58.0	57.1	935-954 bp	1.0	65.6
trnS-GCU-trnG-UCC_p4	CGGAGAGAGAGGGATTCGAA	AACGGAAAGACCCCTTAACT	55.0	45.0	58.3	56.4	982-1001 bp	1.0	65.2
trnS-GCU-trnG-UCC_p5	GCTTTAGTCCACTCAGCCAT	AACGGAAAGACCCCTTAACT	50.0	45.0	57.6	56.4	920-939 bp	1.0	64.5