Hotspot

matK-rps16

LSC · 3288-3954 · 667 bp

Back to Bactris

Pi 0.0025

MarkerSeek score 51.80

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 667
variable_sites: 3
pi: 0.0025
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	AGAAAGTGTTGTTGCCGAGA	GCTCAACCTACGGGAACTG	45.0	57.9	57.7	57.9	804-1764 bp	1.0	68.7
matK-rps16_p2	AGAAAGTGTTGTTGCCGAGA	GTGCTCAACCTACGGGAAC	45.0	57.9	57.7	58.2	806-1766 bp	1.0	68.5
matK-rps16_p3	AGAAAGTGTTGTTGCCGAGA	TGCTCAACCTACGGGAACT	45.0	52.6	57.7	58.2	805-1765 bp	1.0	67.8
matK-rps16_p4	AAGTGTTGTTGCCGAGATCT	GCTCAACCTACGGGAACTG	45.0	57.9	57.4	57.9	801-1761 bp	1.0	66.9
matK-rps16_p5	AAGTGTTGTTGCCGAGATCT	GTGCTCAACCTACGGGAAC	45.0	57.9	57.4	58.2	803-1763 bp	1.0	66.7