Hotspot

petD

LSC · 76226-77442 · 1217 bp

Back to Baccharis

Pi 0.0112

MarkerSeek score 57.10

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1217
variable_sites: 19
pi: 0.0112
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TCCCTTGAACTCCGCAATAT	TGAAGCGACTGTTCCCTAGA	45.0	50.0	56.6	58.1	1352-1407 bp	1.0	69.9
petD_p2	TCCCTTGAACTCCGCAATAT	CTTTGAAGCGACTGTTCCCT	45.0	50.0	56.6	58.1	1355-1410 bp	1.0	69.6
petD_p3	TCCCTTGAACTCCGCAATATT	TGAAGCGACTGTTCCCTAGA	42.9	50.0	57.4	58.1	1352-1407 bp	1.0	67.9
petD_p4	TCCCTTGAACTCCGCAATATT	CTTTGAAGCGACTGTTCCCT	42.9	50.0	57.4	58.1	1355-1410 bp	1.0	67.6
petD_p5	TCCCTTGAACTCCGCAATAT	ACTTTGAAGCGACTGTTCCC	45.0	50.0	56.6	58.4	1356-1411 bp	1.0	67.3