Hotspot

rpl32-ndhF

SSC · 123926-124977 · 1052 bp

Back to Baccharis

Pi 0.0163

MarkerSeek score 71.70

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1052
variable_sites: 24
pi: 0.0163
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	ACGACGCCCAATATCCTTTC	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	58.1	58.0	1220-1299 bp	1.0	83.9
rpl32-ndhF_p2	CGACGCCCAATATCCTTTCA	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	58.1	58.0	1219-1298 bp	1.0	83.9
rpl32-ndhF_p3	ACGACGCCCAATATCCTTTC	TGCCGTCGGAAAAAGAAGAA	50.0	45.0	58.1	58.0	1218-1297 bp	1.0	83.8
rpl32-ndhF_p4	ACGACGCCCAATATCCTTTC	TTGCCGTCGGAAAAAGAAGA	50.0	45.0	58.1	58.0	1219-1298 bp	1.0	83.8
rpl32-ndhF_p5	CGACGCCCAATATCCTTTCA	TGCCGTCGGAAAAAGAAGAA	50.0	45.0	58.1	58.0	1217-1296 bp	1.0	83.8