Hotspot

rpl14-rpl16

LSC · 80785-80904 · 120 bp

Back to Baccharis

Pi 0.0333

MarkerSeek score 73.70

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 120
variable_sites: 6
pi: 0.0333
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ACACATCAATTCTCGAGCCC	AATAGCAGCGTCCAAAATGC	50.0	45.0	58.0	57.4	235-242 bp	1.0	79.3
rpl14-rpl16_p2	TCGACGATTGCTAGCTCCTA	AATAGCAGCGTCCAAAATGC	50.0	45.0	58.0	57.4	265-272 bp	1.0	78.8
rpl14-rpl16_p3	ACACATCAATTCTCGAGCCC	GCGTCCAAAATGCCTATACG	50.0	50.0	58.0	57.3	228-235 bp	1.0	78.6
rpl14-rpl16_p4	TCGACGATTGCTAGCTCCTA	GCGTCCAAAATGCCTATACG	50.0	50.0	58.0	57.3	258-265 bp	1.0	78.1
rpl14-rpl16_p5	CCCGCTGTTATCTGCTACAT	AATAGCAGCGTCCAAAATGC	50.0	45.0	57.5	57.4	217-224 bp	1.0	75.6