Hotspot

infA-rps8

LSC · 79709-79823 · 115 bp

Back to Baccharis

Pi 0.0189

MarkerSeek score 63.20

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 115
variable_sites: 3
pi: 0.0189
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	CGTTGGGAAGCGATTCAGTA	ATTTTAGGTGGGATGGGGGT	50.0	50.0	58.0	58.3	272-281 bp	1.0	81.1
infA-rps8_p2	ATACCGTTGGGAAGCGATTC	ATTTTAGGTGGGATGGGGGT	50.0	50.0	58.1	58.3	276-285 bp	1.0	80.7
infA-rps8_p3	AAACGAACCCGGAACATACC	ATTTTAGGTGGGATGGGGGT	50.0	50.0	58.2	58.3	291-300 bp	1.0	79.4
infA-rps8_p4	CGTTGGGAAGCGATTCAGTA	TTTTAGGTGGGATGGGGGTT	50.0	50.0	58.0	58.5	271-280 bp	1.0	79.3
infA-rps8_p5	CGTTGGGAAGCGATTCAGTA	GTGGGATGGGGGTTGTAATT	50.0	50.0	58.0	57.5	265-274 bp	1.0	79.2