Hotspot

trnW-CCA-trnP-UGG

LSC · 66144-66310 · 167 bp

Back to Baccharis

Pi 0.0162

MarkerSeek score 66.60

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 167
variable_sites: 4
pi: 0.0162
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.0	58.1	229-235 bp	1.0	82.3
trnW-CCA-trnP-UGG_p2	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	50.0	50.0	58.0	58.1	227-233 bp	1.0	82.3
trnW-CCA-trnP-UGG_p3	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	50.0	50.0	58.0	58.1	230-236 bp	1.0	82.3
trnW-CCA-trnP-UGG_p4	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	240-246 bp	1.0	82.2
trnW-CCA-trnP-UGG_p5	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	50.0	50.0	58.0	58.1	241-247 bp	1.0	82.2